| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Rogerson, Annika R (12)
|
VAUN-V |
27.81 |
27.84 |
0.03 |
| 2 |
Ismailova, Diana (11)
|
VAGATR |
27.55 |
27.85 |
0.30 |
| 3 |
Czirjak, Sara E (12)
|
VAGATR |
29.43 |
28.53 |
-0.90 |
| 4 |
Segura, Mara J (11)
|
VATSU |
33.66 |
30.96 |
-2.70 |
| 5 |
Hoang, Angelina (11)
|
VAACAC |
|
30.99 |
- |
| 6 |
McCardle, Reese B (11)
|
VAACAC |
32.57 |
31.07 |
-1.50 |
| 7 |
Dugas, Georgia K (12)
|
VAACAC |
33.69 |
31.12 |
-2.57 |
| 8 |
Brushwood, Madison P (11)
|
VABASS |
34.84 |
32.56 |
-2.28 |
| 9 |
Turner, Paige Y (11)
|
VATSU |
34.55 |
32.95 |
-1.60 |
| 10 |
Barclay, Sydney M (12)
|
VAACAC |
35.46 |
33.33 |
-2.13 |
| 11 |
Wells, Abigail J (11)
|
VATSU |
|
33.43 |
- |
| 12 |
Semmelhack, Sylvie W (11)
|
VAGATR |
|
34.54 |
- |
| 13 |
Hernandez, Maya G (11)
|
VABASS |
45.80 |
34.61 |
-11.19 |
| 14 |
Brooks, Madelyne G (11)
|
VABASS |
40.41 |
36.07 |
-4.34 |
| 15 |
McCORKLE, Madeline G (11)
|
VATSU |
|
36.72 |
- |
| 16 |
Brice, Madison R (11)
|
VABASS |
|
42.35 |
- |
| 17 |
Mejeur, Hannah J (12)
|
VATSU |
1:09.67 |
44.07 |
-25.60 |
| 18 |
Martin, Grace E (11)
|
VABASS |
|
48.56 |
- |